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by Olwyn M. R. Westwood,Frank C. Hay

Download Epitope Mapping: A Practical Approach (Practical Approach Series) eBook
Olwyn M. R. Westwood,Frank C. Hay
Medicine & Health Sciences
Oxford University Press; 1 edition (May 24, 2001)
304 pages
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Hay, Oxford University Press, 2000, 284 p.

Hay, Oxford University Press, 2000, 284 p. Article in Biochimie 83(10):986–987 · October 2001 with 13 Reads. DOI: 1. 016/S030084(01)01336-0.

Epitope Mapping book. Epitope Mapping: A Practical Approach (Practical Approach Series, 248). 0199636532 (ISBN13: 9780199636532).

Frank C. Hay, Olwyn . The definitive lab "bench book" A one stop resource Techniques explained from first principles Basic forms of apparatus described in detail Totally revised with new user friendly layout to aid use in the lab Includes useful hints and tips.

Epitope mapping can reveal how a mAb exerts its functional effects - for instance, by blocking the binding of a ligand or by. Westwood, Olwyn M. Hay, Frank . eds. (2001). Epitope Mapping: A Practical Approach.

Epitope mapping can reveal how a mAb exerts its functional effects - for instance, by blocking the binding of a ligand or by trapping a protein in a non-functional state. Many therapeutic mAbs target conformational epitopes that are only present when the protein is in its native (properly folded) state, which can make epitope mapping challenging. Epitope mapping has been crucial to the development of vaccines against prevalent or deadly viral pathogens, such as chikungunya, dengue, Ebola, and Zika viruses, by determining.

Epitope mapping: a practical approach. Characterisation of anti-IgG monoclonal antibody A57H by epitope mapping. Pn nelson, om westwood, roy jefferis, M goodall, FC hay. OMR Westwood, FC Hay. Oxford University Press, USA, 2001. N-terminal sequence analysis of human placental protein 14, purified in high yield from decidual cytosol. OMR Westwood, MG Chapman, N Totty, R Philp, AE Bolton, NR Lazarus. Reproduction 82 (2), 493-500, 1988. Biochemical Society Transactions 25 (2), 373S-373S, 1997. The generation of diversity. Immunology, London, Mosby International, 93-106, 1998.

Epitope mapping : a practical approach

Epitope mapping : a practical approach. Olwyn M. R. Westwood, Frank C. Hay. 2000. Introduction to epitope mapping Synthetic peptides I pepscan assay to define antigenic determinants for antibodies (B cell Epitopes) Delineation of helper T cell epitopes and MHC Combined T and . More). Phagocytosis, Complement and Antibodydependent Cytotoxicity. Frank C. Hay, Olwyn M. Westwood.

The Practical approach series. Bibliography, etc. Note: Includes bibliographical references and index. Personal Name: Westwood, Olwyn M. Personal Name: Hay, Frank C. Rubrics: Antigenic determinants Laboratory manuals. by Pompeij bakom ruinernaHans Furuhagen. Natur och Kultur (c)2004.

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Epitope Mapping covers all the major methods for the identification and definition of epitopes. The Pepscan assay is used to define B cell epitopes and makes use of synthetic peptides but can only be used if the amino acid sequence is known. It can be adapted for the delineation of both helper T cells and cytotoxic T cells. The identification of combined B and T cell epitopes can also be achieved using synthetic peptides. There are other methodologies for analyzing for cytotoxic T cell epitopes such as the purification of antigens presented by MHC class I molecules and expression cloning. Site directed mutagenesis is also a powerful tool in epitope mapping and can be used to evaluate the role of single amino acids in immune complex formation. Protein footprinting makes use of monoclonal antibodies produced by hybridoma technology and relies on the fact that the epitope is protected from cleavage when bound as an antibody-antigen complex. It is only useful for small antigens. Other monoclonal antibody assays such as enzyme linked immunosorbent assay and haemaglutination and slot-blotting may also be used in epitope mapping. Random phage display libraries bring together the genetic and amino acid peptide sequence and can be screened with antibody and the resulting peptide DNA sequenced to confirm the amino acid sequence of a specific epitope. Investigation of carbohydrates can also be useful to epitope mapping as deglycosylation can lead to loss of antigenic activity. Epitopes are important to the pharmaceutical industry and wherever appropriate, pharmaceutical applications of the methods described are included. For each method there is a description of the technology, protocols, trouble-shooting, and advice on when to use the method. This book will therefore be invaluable to any researcher involved in epitope mapping.